The Adhesion Activity and Inhibition of Adhesion Klebsiella pneumoniae Strains by Lactobacillus bulgaricus in Soyghurt in In-Vitro on HEp-2 Cell Lines with Preinfection Treatment

JETTY NURHAJATI^1*., CHRYSANTI²,  PRIMA NANDA FAUZIAH<sup>1

1</sup> Biology Department, Faculty of Mathematic and Natural Science, Padjadjaran University, Indonesia.
²Microbiology Department, Faculty of Medicine, Padjadjaran University, Indonesia
^*Email: primayaa@yahoo.com

ABSTRACT

Attachment or adhesion of Lactobacillus bulgaricus in soyghurt and Klebsiella pneumoniae strains and also inhibition of adhesion K.pneumoniae in the respiratory tract by using L.bulgaricus has been done in the laboratory using HEp-2 cell lines with preinfection treatment. This research was aimed at finding out the adhesion ability of L.bulgaricus in soyghurt and K.pneumoniae strains, and also to force anti-adhesives L.bulgaricus on the adhesion of K.pneumoniae strains to HEp-2 cell lines. This research consists of two phases: first, examine the adhesion ability of L.bulgaricus in soyghurt and K.pneumoniae strains on HEp-2 cell lines. The second phase was testing the concentration of L.bulgaricus in soyghurt for inhibiting the adhesion of K.pneumoniae strains on HEp-2 cell lines according to the contact time of infection. Results of the first phase showed that L.bulgaricus in soyghurt could carry out adherence in HEp-2 cell lines after 5 hours of contact with HEp-2 cell lines by expressing diffuse adherence (DA), whereas K.pneumoniae strains could carry out adherence on HEp-2 cell lines after 5 h of contact with HEp-2 cell lines by expressing aggregative adherence (AA). The second phase of the research results showed the treatment of L.bulgaricus in soyghurt with concentration 108 CFU/ml can decreased the adhesion percentage of K.pneumoniae strains to HEp-2 cell lines after 5 h on the pre infection. Percentage total of K.pneumoniae ATCC 700603 only can be adhesion on HEp-2 cell lines amounting 6.42% compared to the controls of K.pneumoniae ATCC 700603 to wit 88.19%, whereas for percentage total of K.pneumoniae CT1538 and K.pneumoniae S941 can be adhesion amounting 30% and 10,11%. L.bulgaricus in soyghurt has adhesive characteristics, so it can be expected to carry out adherence in respiratory tract and could inhibit K.pneumoniae adhesion activity.

Keywords: Klebsiella pneumoniae, Lactobacillus bulgaricus, Bacteria Adhesion, Soyghurt. 

 Has been published on 8th National Congress of Indonesia Association of Clinical Microbiology (PAMKI) (November -3th, 2012)