PRIMA NANDA FAUZIAH1*, JETTY NURHAJATI1, CHRYSANTI2
.
1Biology Department, Faculty of Mathematic and Natural
Science, Padjadjaran University, Indonesia.
2Microbiology Department, Faculty of Medicine,
Padjadjaran University, Indonesia
*Email:
primayaa@yahoo.com
ABSTRACT
An attempt to produce of natural antibiotic with the
effectiveness of lactic acid filtrate and bacteriocins of L.bulgaricus in soyghurt according of concentration against
inhibitory zone growth of K.pneumoniae
strains has been done in laboratory. This research aimed to get the best
treatment among concentration of lactic acid filtrate and bacteriocins of L.bulgaricus in soyghurt against the MIC
(Minimum Inhibitory Concentration)
value and growth inhibition zones of K.pneumoniae
strains. This research consists of two phases: first examine the MIC of lactic
acid filtrate and bacteriocins of L.bulgaricus
in soyghurt against K.pneumoniae
strains. The second phase is testing the effectiveness of lactic acid filtrate
and bacteriocins of L.bulgaricus in
soyghurt against inhibitory zones growth of K.pneumoniae
strains. Results of the first research showed the concentration 20% of lactic
acid filtrate and 50% bacteriocins of L.bulgaricus
in soyghurt can bactericidal tend against K.pneumoniae
strains. The second phases of the research results showed the effectiveness of lactic
acid filtrate and bacteriocins of L.bulgaricus
in soyghurt can gave the effect against increasing of inhibition zones of K.pneumoniae strains. The greatest of
inhibition zones obtained K.pneumoniae
ATCC 700603 in the concentration 90% of lactic
acid filtrate amounting 15,667 mm. The inhibitory zone growth of K.pneumoniae strains depending on the
concentration of L.bulgaricus in
soyghurt, and bacteria strains.
Key words
: Klebsiella pneumoniae, Lactobacillus bulgaricus, MIC, Filtrat Effectiveness, Soyghurt.
Has been published on 8th National Congress of Indonesia Association of Clinical Microbiology (PAMKI) (November -3th, 2012)
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