4.3.13

The Effectiveness of Lactic Acid Filtrate and Bacteriocins of Lactobacillus bulgaricus KS1 Strain Against the Growth of Klebsiella pneumoniae ATCC 700603, CT1538 and S941 Strains



PRIMA NANDA FAUZIAH1*, JETTY NURHAJATI2, CHRYSANTI3
.
1Microbiology Laboratory, Health Analyst Department, Stikes Jenderal Achmad Yani, Indonesia
2Biology Department, Faculty of Mathematic and Natural Science, Padjadjaran University, Indonesia.
3Microbiology Department, Faculty of Medicine, Padjadjaran University, Indonesia
*Email: primanandafauziah@analis-ayani.ac.id

ABSTRACT

            An attempt to produce a natural antibiotic with the effectiveness of lactic acid filtrate and bacteriocins of L.bulgaricus KS1 strain against the growth of K.pneumoniae ATCC 700603, CT1538 and S941 strains has been done in laboratory. This research was aimed to get the best treatment among concentration of lactic acid filtrate and bacteriocins of L.bulgaricus against the MIC (Minimum Inhibitory Concentration) value and growth inhibition zones of K.pneumoniae strains. This research consisted of two phases: first examine the MIC of lactic acid filtrate and bacteriocins of L.bulgaricus against K.pneumoniae strains. The second phase is testing the effectiveness of lactic acid filtrate and bacteriocins of L.bulgaricus against inhibitory zones growth of K.pneumoniae strains. The research used experimental method in laboratory. The MIC test of L.bulgaricus filtrate against the growth of K.pneumoniae strains used agar method, whereas the effectiveness test of lactic acid filtrate and bacteriocins of L.bulgaricus against inhibitory zones growth of K.pneumoniae strains used diffuse (paper dish) method. Results of the first research showed that the concentration of 30% lactic acid filtrate and 20% bacteriocins of L.bulgaricus could give bactericidal effect against K.pneumoniae strains. The second phases of the research resulted the lactic acid filtrate and bacteriocins of L.bulgaricus could increase the inhibition zones of K.pneumoniae strains. The greatest of inhibition zones obtained K.pneumoniae ATCC 700603 in the concentration of 90% bacteriocins filtrate amounting 16,667 mm. The inhibitory zone growth of K.pneumoniae strains depending on the concentration of L.bulgaricus filtrate, kind of L.bulgaricus filtrate and bacteria strains.

Key words       : Klebsiella pneumoniae, Lactobacillus bulgaricus, MIC, Bacteriocins, Lactic acid, Effectiveness.

 Has been published on 4th International Conference - 
Indonesian Society For Lactic Acid Bacteria  (ISLAB) 
(January -25th-26th, 2013)

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