PRIMA NANDA FAUZIAH1*, JETTY NURHAJATI2, CHRYSANTI3
.
1Microbiology Laboratory, Health
Analyst Department, Stikes Jenderal Achmad Yani, Indonesia
2Biology Department, Faculty of
Mathematic and Natural Science, Padjadjaran University, Indonesia.
3Microbiology Department, Faculty
of Medicine, Padjadjaran University, Indonesia
*Email: primanandafauziah@analis-ayani.ac.id
ABSTRACT
An
attempt to produce a natural
antibiotic with the effectiveness of lactic acid filtrate and bacteriocins of L.bulgaricus KS1 strain against the growth of K.pneumoniae ATCC 700603, CT1538 and S941 strains has been done in
laboratory. This research was
aimed
to get the best treatment among concentration of lactic acid filtrate and
bacteriocins of L.bulgaricus against
the MIC (Minimum Inhibitory Concentration)
value and growth inhibition zones of K.pneumoniae
strains. This research consisted
of two phases: first examine the MIC of lactic acid filtrate and bacteriocins
of L.bulgaricus against K.pneumoniae strains. The second phase
is testing the effectiveness of lactic acid filtrate and bacteriocins of L.bulgaricus against inhibitory zones
growth of K.pneumoniae strains. The research used experimental method in laboratory.
The MIC test of L.bulgaricus filtrate against the growth of K.pneumoniae
strains used agar method, whereas the effectiveness test of lactic acid
filtrate and bacteriocins of L.bulgaricus against inhibitory zones growth
of K.pneumoniae strains used diffuse (paper dish) method.
Results of the first research showed that the concentration of 30% lactic acid filtrate and 20%
bacteriocins of L.bulgaricus could give bactericidal effect against K.pneumoniae strains. The second phases
of the research resulted
the lactic acid filtrate and bacteriocins of L.bulgaricus could
increase the inhibition zones of K.pneumoniae strains. The greatest of
inhibition zones obtained K.pneumoniae
ATCC 700603 in the concentration of
90%
bacteriocins filtrate amounting 16,667 mm. The inhibitory zone growth of K.pneumoniae strains depending on the
concentration of L.bulgaricus
filtrate, kind of L.bulgaricus
filtrate and bacteria strains.
Key words
: Klebsiella pneumoniae, Lactobacillus bulgaricus, MIC, Bacteriocins, Lactic acid, Effectiveness.
Has been published on 4th International Conference -
Indonesian Society For Lactic Acid Bacteria (ISLAB)
(January -25th-26th, 2013)
Indonesian Society For Lactic Acid Bacteria (ISLAB)
(January -25th-26th, 2013)
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